HESI RN
Biology Test
1. Which of the following statements is most accurate regarding the transcription of DNA?
- A. RNA uses reverse transcriptase to make mRNA
- B. DNA uses mRNA to produce tRNA
- C. RNA creates a duplicate copy of DNA
- D. RNA copies DNA to produce mRNA
Correct answer: D
Rationale: The correct answer is D. During transcription, RNA copies DNA to produce mRNA, which carries the genetic information from DNA to the ribosomes for protein synthesis. Choice A is incorrect because reverse transcriptase is not involved in the transcription of DNA. Choice B is incorrect as DNA does not use mRNA to produce tRNA; tRNA is produced through a different process. Choice C is incorrect as RNA does not create a duplicate copy of DNA during transcription; it synthesizes mRNA by copying the DNA template.
2. Which of the following organelles is the site of cellular respiration?
- A. Nucleus
- B. Mitochondria
- C. Lysosomes
- D. Ribosomes
Correct answer: B
Rationale: The correct answer is B: Mitochondria. Mitochondria are known as the powerhouse of the cell because they generate most of the cell's supply of ATP through cellular respiration. The other organelles listed, such as the nucleus (choice A), lysosomes (choice C), and ribosomes (choice D), do not play a direct role in cellular respiration. The nucleus contains genetic material, lysosomes are involved in digestion and waste removal, and ribosomes are responsible for protein synthesis.
3. Prophase is the first stage of mitosis. Which of the following best describes chromosomes during this stage?
- A. They are gathered on either side of the separating cell
- B. They are all aligned along the metaphase plate
- C. They are attached to the spindle
- D. They are visibly separate
Correct answer: D
Rationale: The correct answer is D. During prophase, the chromosomes condense and become visible as distinct structures under a microscope. Choice A is incorrect as chromosomes are not gathered on either side of the separating cell during prophase. Choice B is incorrect because chromosomes align along the metaphase plate during the subsequent stage, metaphase. Choice C is incorrect as chromosomes become attached to the spindle fibers during prometaphase, the stage following prophase.
4. Which of the following options is the term used to represent alternative versions of a gene?
- A. Alleles
- B. Binary fission
- C. Heterozygous
- D. Homozygous
Correct answer: A
Rationale: The correct answer is A: Alleles. Alleles are different forms of a gene that can exist at a specific locus on a chromosome. They represent variations in a gene's DNA sequence. Choices B, C, and D are incorrect. Binary fission is a form of asexual reproduction in single-celled organisms, while heterozygous and homozygous refer to different combinations of alleles in an individual, not the alternative versions of a gene.
5. What is located on the rough endoplasmic reticulum?
- A. Microvilli
- B. Mitochondria
- C. Lysosomes
- D. Ribosomes
Correct answer: D
Rationale: The rough endoplasmic reticulum is studded with ribosomes, which are responsible for protein synthesis. Ribosomes are the correct answer because they are the organelles directly associated with the rough endoplasmic reticulum. Microvilli (choice A), mitochondria (choice B), and lysosomes (choice C) are not typically located on the rough endoplasmic reticulum. Microvilli are found on the surface of cells to increase surface area, mitochondria are the powerhouses of the cell responsible for energy production, and lysosomes are membrane-bound organelles containing digestive enzymes.
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