Nursing Elites

1. Which of the following is directly transcribed from DNA and represents the first step in protein synthesis?

• A. siRNA
• B. rRNA
• C. mRNA
• D. tRNA

Correct answer: C

Rationale: mRNA (messenger RNA) is directly transcribed from DNA in the nucleus during the process of transcription. It serves as a template for protein synthesis during translation in the cytoplasm. mRNA carries genetic information from the DNA to the ribosomes, where it determines the sequence of amino acids in a protein. Thus, mRNA represents the first step in protein synthesis as it carries the code from the DNA to be translated into a protein. Choice A, siRNA (small interfering RNA), is involved in post-transcriptional gene silencing and does not play a role in protein synthesis. Choice B, rRNA (ribosomal RNA), is a component of the ribosome and plays a structural and functional role in protein synthesis but is not directly transcribed from DNA. Choice D, tRNA (transfer RNA), is responsible for bringing amino acids to the ribosome during protein synthesis but is not directly transcribed from DNA.

2. Which type of reaction builds larger and more complex molecules from smaller ones, requiring energy?

• A. Catabolic reaction
• B. Anabolic reaction
• C. Exothermic reaction
• D. Hydrolytic reaction

Correct answer: B

Rationale: The correct answer is 'Anabolic reaction.' Anabolic reactions involve the synthesis of larger and more complex molecules from smaller ones, requiring an input of energy, often in the form of ATP. These reactions are essential for processes like growth, repair, and the building of cellular structures. Catabolic reactions, on the other hand, break down larger molecules into smaller ones, releasing energy. Exothermic reactions release heat, while hydrolytic reactions involve the breakdown of molecules with the addition of water. Therefore, choices A, C, and D are incorrect as they do not describe the process of building larger and more complex molecules from smaller ones with the requirement of energy.

3. What is the primary function of the pyloric sphincter?

• A. to regulate the movement of food material from the stomach to the duodenum
• B. to neutralize stomach acid
• C. to prevent digested food materials and stomach acid from entering the esophagus
• D. to begin the process of chemical digestion

Correct answer: A

Rationale: The primary function of the pyloric sphincter is to regulate the flow of partially digested food material (chyme) from the stomach into the small intestine, specifically the duodenum. This control is essential for proper digestion and absorption of nutrients in the small intestine. Choices B, C, and D are incorrect. Choice B is incorrect because neutralizing stomach acid is primarily the function of the stomach lining and antacid mechanisms. Choice C is incorrect because preventing the backflow of digested food materials and stomach acid into the esophagus is mainly the role of the lower esophageal sphincter. Choice D is incorrect because the chemical digestion process primarily starts in the stomach through the action of gastric juices, not the pyloric sphincter.

4. How can bacteria acquire new genetic material from their environment?

• A. Transformation
• B. Transduction
• C. Conjugation
• D. All of the above

Correct answer: D

Rationale: A) Transformation: Transformation is the process by which bacteria can take up free DNA from their environment and incorporate it into their own genome, leading to the acquisition of new genetic material and traits. B) Transduction: Transduction involves the transfer of genetic material from one bacterium to another by a bacteriophage, a virus that infects bacteria. The bacteriophage carries bacterial DNA from one host cell to another, facilitating the transfer of genetic material. C) Conjugation: Conjugation is a mechanism of horizontal gene transfer in bacteria where genetic material is transferred between two bacterial cells in direct contact. This transfer is facilitated by a conjugative plasmid carrying the genetic information. Therefore, all the processes mentioned (transformation, transduction, and conjugation) are ways in which bacteria can acquire new genetic material from their environment.

5. What type of bond holds water molecules together?

• A. Covalent bond
• B. Hydrogen bond
• C. Ionic bond
• D. Peptide bond

Correct answer: B

Rationale: The correct answer is B: Hydrogen bond. Hydrogen bonds are responsible for holding water molecules together. In a water molecule, the oxygen atom is slightly negative, and the hydrogen atoms are slightly positive, creating a partial positive and negative charge. This polarity allows hydrogen bonds to form between adjacent water molecules. Covalent bonds involve the sharing of electrons, ionic bonds involve transfer of electrons between ions, and peptide bonds are specific to linking amino acids in proteins, which are not relevant to water molecule interactions.

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