what is the crispr cas9 system used for in gene editing

ATI TEAS 7

TEAS Test 7 science quizlet

1. What is the primary function of the CRISPR-Cas9 system in gene editing?

A. To amplify specific DNA sequences.
B. To cut DNA at specific locations.
C. To deliver genes into cells.
D. To sequence entire genomes.

Correct answer: B

Rationale: The CRISPR-Cas9 system is a powerful tool used in gene editing to precisely target and cut DNA at specific locations within the genome. This system consists of a guide RNA that directs the Cas9 enzyme to the desired DNA sequence, where it creates a double-strand break. This break can then be repaired by the cell's natural DNA repair mechanisms, allowing for the introduction of specific genetic modifications. Options A, C, and D are incorrect as they do not accurately describe the primary function of the CRISPR-Cas9 system in gene editing. Amplifying DNA sequences, delivering genes into cells, and sequencing entire genomes are not the main functions of the CRISPR-Cas9 system, which is primarily focused on precise DNA cutting for targeted genetic modifications.

2. Which of the following statements is NOT true about DNA?

A. DNA contains the nucleotides A, T, C, G and is read 5' to 3'.
B. DNA contains genetic information and is tightly coiled with proteins to form chromosomes.
C. DNA contains the nucleotides A, T, C, G and is read 3' to 5'.
D. DNA is a double-stranded molecule connected by hydrogen bonds.

Correct answer: C

Rationale: The correct answer is C because DNA is typically read from the 5' end to the 3' end, not from 3' to 5'. Choices A, B, and D are all true statements about DNA. DNA contains the nucleotides A, T, C, G and is read in a 5' to 3' direction. DNA also contains genetic information and is tightly coiled with proteins to form chromosomes. Additionally, DNA is a double-stranded molecule connected by hydrogen bonds.

3. Which statement most accurately compares and contrasts the structures of DNA and RNA?

A. Both DNA and RNA have 4 nucleotide bases. Three of the bases are the same, but the fourth base is thymine in DNA and uracil in RNA.
B. Both DNA and RNA have the same 4 nucleotide bases. However, the nucleotides bond differently in DNA compared to RNA.
C. Both DNA and RNA have different numbers of nucleotide bases. DNA is a double helix while RNA is a single strand.
D. Both DNA and RNA have a double helix structure. However, DNA contains 6 nucleotide bases and RNA contains 4 nucleotide bases.

Correct answer: A

Rationale: The correct answer is A because DNA and RNA both consist of 4 nucleotide bases, namely adenine, cytosine, and guanine, which are common in both. The key difference lies in the fourth base, which is thymine in DNA and uracil in RNA. This distinction is fundamental in understanding the structural variances between DNA and RNA. Choice B is incorrect as it incorrectly states that the nucleotides bond differently in DNA compared to RNA without specifying the crucial base differences. Choice C is inaccurate as it misrepresents the numbers of nucleotide bases in DNA and RNA, and incorrectly describes DNA as a double helix and RNA as a single strand, which is not related to the difference in bases. Choice D is incorrect as it inaccurately portrays DNA with 6 nucleotide bases and RNA with 4, which is not true; both DNA and RNA have 4 nucleotide bases, but with differences in one of the bases.

4. Which property of matter remains constant regardless of changes in its state?

A. Mass
B. Volume
C. Density
D. Weight

Correct answer: A

Rationale: The correct answer is 'Mass.' Mass is a measure of the amount of matter in an object and remains constant regardless of changes in its state. When matter changes its state (solid, liquid, gas), its mass remains the same. On the other hand, volume can change with the shape the matter takes, density changes as the mass is distributed differently, and weight can vary with the gravitational pull. Therefore, mass is the property that remains constant irrespective of the state of matter, making it the correct choice in this scenario.

5. How does the Law of Conservation of Mass apply to this reaction: 2H₂ + O₂ → 2H₂O?

A. Electrons are not lost.
B. The hydrogen does not lose mass.
C. New water molecules are formed.
D. There is no decrease or increase in matter.

Correct answer: D

Rationale: The Law of Conservation of Mass states that matter cannot be created or destroyed in a chemical reaction. In the given reaction, 2 moles of hydrogen combine with 1 mole of oxygen to form 2 moles of water. The total mass of the reactants (hydrogen and oxygen) is equal to the total mass of the products (water), meaning there is no decrease or increase in matter. The total mass of the system remains constant, demonstrating the conservation of mass. Choices A, B, and C are incorrect because the conservation of mass does not specifically relate to electrons, individual elements (like hydrogen), or the formation of new molecules; instead, it focuses on the overall mass of the system before and after the reaction.