HESI A2
Biology HESI A2 Practice Test
1. Which part of cellular respiration produces the greatest amount of ATP?
- A. electron transport chain
- B. glycolysis
- C. citric acid cycle
- D. fermentation
Correct answer: A
Rationale: The electron transport chain (ETC) produces the greatest amount of ATP during cellular respiration. This process occurs in the inner mitochondrial membrane and involves the transfer of electrons through a series of protein complexes, creating a proton gradient that drives the synthesis of ATP. By utilizing the energy from the electron carriers NADH and FADH2 produced in earlier stages of cellular respiration, the ETC can generate a large amount of ATP efficiently through oxidative phosphorylation. Glycolysis only produces a small amount of ATP in comparison to the ETC. The citric acid cycle generates some ATP but not as much as the ETC. Fermentation does not produce ATP through oxidative phosphorylation and yields a much smaller amount of ATP compared to the ETC.
2. Which organelle would you expect to be present in a cell responsible for detoxifying multiple molecules?
- A. rough ER
- B. smooth ER
- C. lysosome
- D. Golgi apparatus
Correct answer: B
Rationale: The smooth endoplasmic reticulum (ER) is the organelle responsible for detoxifying multiple molecules in a cell. It contains enzymes that help break down toxins and drugs, making it an essential organelle for detoxification processes. The rough ER, lysosome, and Golgi apparatus do not play direct roles in detoxification. The rough ER is mainly involved in protein synthesis and transport. Lysosomes are responsible for digestion and recycling within the cell. The Golgi apparatus is involved in modifying, sorting, and packaging proteins for secretion or for use within the cell.
3. Which of the following is necessary for cell diffusion?
- A. water
- B. membrane
- C. ATP
- D. gradient
Correct answer: D
Rationale: The correct answer is 'gradient.' In cell diffusion, substances move from an area of high concentration to an area of low concentration, driven by the concentration gradient. This process does not require water, membrane, or ATP specifically. Water can aid in the movement of substances, but it is not necessary for diffusion itself. The membrane is involved in regulating diffusion but is not a requirement for the process. ATP is not directly involved in simple diffusion processes; instead, it is more related to active transport mechanisms. Therefore, the concentration gradient is essential for cell diffusion to occur.
4. The procedure to focus an image using a compound microscope involves
- A. Adjusting the coarse adjustment each time a new magnification is selected
- B. Adjusting the fine adjustment each time a new magnification is selected
- C. Adjusting the stage each time a new magnification is selected
- D. All of the above
Correct answer: B
Rationale: When using a compound microscope, it is essential to adjust the fine adjustment knob each time you switch to a new magnification level. The fine adjustment knob allows for precise focusing on the specimen at different magnifications, ensuring a clear and sharp image. The coarse adjustment knob is typically used only at the lower magnification settings to bring the image into view roughly. Adjusting the stage may be necessary based on the size and thickness of the specimen, but it does not need to be done every time a new magnification is selected. Therefore, option B is the correct choice as it focuses on the key aspect of precise focusing during magnification changes.
5. What happens to glucose during glycolysis?
- A. Its energy is entirely lost.
- B. It splits into molecules of pyruvic acid.
- C. It is stored in NADH.
- D. It joins with molecules of citric acid.
Correct answer: B
Rationale: During glycolysis, glucose undergoes a series of enzymatic reactions in the cytoplasm of the cell, resulting in its breakdown into two molecules of pyruvic acid. This process also generates ATP and NADH as energy carriers. Choice A is incorrect because glucose is not entirely lost, but rather converted into other molecules. Choice C is incorrect because NADH is a product of glycolysis, not a storage form for glucose. Choice D is incorrect as glucose does not join with molecules of citric acid during glycolysis, but rather in subsequent stages of cellular respiration.
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